Measurement

Part:BBa_K1316003:Design

Designed by: Joan Cortada Garcia   Group: iGEM14_TU_Delft-Leiden   (2014-08-24)


yqjF promoter coupled to mKate2 reporter gene


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 128
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 733
    Illegal BsaI.rc site found at 922


Design Notes

The yqjF promoter carries 4 point mutations to improve its response in front of 2,4,6-TNT and 2,4-DNT


Source

Combination of K1316002 with K1316001

References